The fission yeast rad22 gene, having a function in mating-type switching and repair of DNA damages, encodes a protein homolog to Rad52 of Saccharomyces cerevisiae

Nucleic Acids Res. 1993 Dec 25;21(25):5940-4. doi: 10.1093/nar/21.25.5940.


The gene rad22 of the fission yeast Schizosaccharomyces pombe has a function in DNA repair and mating-type switching. We have cloned the rad22 gene from a genomic gene bank by functional complementation of the switching defect. An open reading frame coding for a putative protein of 469 amino acids was found by sequence analyses. The rad22 gene contains no intron. A region of 126 amino acids in the N-terminal half of the Rad22 protein has significant homologies (56% identity and 36% similarity) to the Rad52 protein of Saccharomyces cerevisiae. A rad22 disruption strain was constructed which seems to be inviable in a homothallic background. Southern blot analyses have shown that the rad22-67 mutant frequently gives rise to deletions in the mating-type region. These data indicate that the Rad22 protein has a function in the repair of DNA double-strand breaks.

MeSH terms

  • Amino Acid Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA Damage
  • DNA Repair / genetics*
  • DNA, Fungal / genetics
  • DNA-Binding Proteins / genetics*
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Genes, Mating Type, Fungal*
  • Genes, Switch
  • Molecular Sequence Data
  • Rad52 DNA Repair and Recombination Protein
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins*
  • Sequence Homology, Amino Acid


  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • RAD52 protein, S cerevisiae
  • Rad52 DNA Repair and Recombination Protein
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • rad52 protein, S pombe

Associated data

  • GENBANK/X72220