Characterization of an early activation-dependent antigen on lymphocytes defined by the monoclonal antibody BL-Ac(F2)

Scand J Immunol. 1994 Jan;39(1):111-5. doi: 10.1111/j.1365-3083.1994.tb03348.x.


A novel activation-dependent lymphocyte cell-surface antigen which is recognized by a MoAb, BL-Ac(F2), is described. Although not found on resting lymphocytes the antigen is induced rapidly within 2-4 h following stimulation of the cells using mitogens or antibodies against the T-cell CD3 antigen and sIgM on B cells, respectively. Immunoprecipitation and Western Blotting indicated that the MoAb recognizes a molecule in a range of 78-85 kDa. Beyond its activation-dependent expression on lymphocytes the antigen was detected also on myelo-monocytic cells. Expression kinetics and cellular distribution of this molecule suggest that it is distinct from previously described activation-dependent cell-surface antigens such as CD69, CD25 and 4F2.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigens, CD / immunology
  • Antigens, Differentiation, T-Lymphocyte / immunology
  • Antigens, Surface / immunology
  • Blotting, Western
  • Cell Line
  • Humans
  • Lectins, C-Type
  • Lymphocyte Activation / immunology
  • Lymphocytes / immunology*
  • Mice
  • Mitogens
  • Precipitin Tests
  • Receptors, Interleukin-2 / immunology
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • CD69 antigen
  • Lectins, C-Type
  • Mitogens
  • Receptors, Interleukin-2