Proteoglycans and Endothelial Barrier Function: Effect of Linoleic Acid Exposure to Porcine Pulmonary Artery Endothelial Cells

Atherosclerosis. 1993 Nov;103(2):279-90. doi: 10.1016/0021-9150(93)90270-5.


Certain fatty acids induce changes in endothelial barrier function which may be mediated by alterations in normal proteoglycan synthesis/metabolism. To test this hypothesis, pulmonary artery derived endothelial cells were treated with media supplemented with linoleic acid (18:2), and/or a known proteoglycan synthesis inhibitor, beta-D-xyloside. Independent exposure to 1 mM beta-D-xyloside or 90 microM 18:2 increased albumin transfer, i.e., decreased barrier function, when compared with control cultures. 18:2 and beta-D-xyloside increased albumin transfer additively, suggesting that the mechanisms by which 18:2 and beta-D-xyloside alter the proteoglycan metabolism are different. Compared with the control group, treatment with 18:2 inhibited proteoglycan synthesis, decreased anionic properties of heparan sulfate proteoglycans in the cell monolayers and caused the release of a unique chondroitin sulfate proteoglycan into the culture media. Treatment with beta-D-xyloside caused an increased incorporation of radioactive sulfate into glycosaminoglycans but inhibited proteoglycan synthesis. These results suggest that the fatty acid- and beta-D-xyloside-induced impairment in endothelial barrier function may involve changes in the synthesis, release and physicochemical properties of proteoglycans.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Albumins / metabolism
  • Animals
  • Biological Transport
  • Cells, Cultured
  • Chromatography, Ion Exchange
  • Dimethyl Sulfoxide / pharmacology
  • Dose-Response Relationship, Drug
  • Endothelium, Vascular / metabolism*
  • Glycosides / pharmacology
  • Linoleic Acid
  • Linoleic Acids / pharmacology*
  • Proteoglycans / metabolism*
  • Pulmonary Artery / metabolism*
  • Swine


  • Albumins
  • Glycosides
  • Linoleic Acids
  • Proteoglycans
  • xylosides
  • Linoleic Acid
  • Dimethyl Sulfoxide