Introduction of antisense oligonucleotides into cells by permeabilization with streptolysin O

Biotechniques. 1993 Dec;15(6):1016-8, 1020.


Cellular uptake of antisense oligonucleotides is critical to their ability to inhibit gene expression. In the present study, phosphodiester oligodeoxynucleotides were introduced into cells during brief permeabilization with the pore-forming agent streptolysin O. The extent of antisense inhibition was dependent on the concentration of oligonucleotide present during permeabilization. In addition, the level of antisense inhibition was time-dependent; it reached a maximum at 18 h and subsequently diminished to control levels over the next 48 h. These results demonstrate the effectiveness of streptolysin O permeabilization as a means for simple and rapid introduction of oligonucleotides into eukaryotic cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Technical Report

MeSH terms

  • Animals
  • Bacterial Proteins
  • Base Sequence
  • Calcium / metabolism
  • Calcium Channels / genetics*
  • Cell Line, Transformed
  • Cell Membrane Permeability / drug effects*
  • Chlorothiazide / pharmacology
  • Kidney Tubules, Distal / drug effects
  • Kidney Tubules, Distal / metabolism
  • Kinetics
  • Mice
  • Molecular Sequence Data
  • Oligonucleotides, Antisense / metabolism*
  • Streptolysins / pharmacology*


  • Bacterial Proteins
  • Calcium Channels
  • Oligonucleotides, Antisense
  • Streptolysins
  • streptolysin O
  • Chlorothiazide
  • Calcium