The DNA polymerase-encoding gene of African swine fever virus: sequence and transcriptional analysis

Gene. 1993 Dec 22;136(1-2):103-10. doi: 10.1016/0378-1119(93)90453-a.


The putative DNA polymerase-encoding gene of African swine fever virus has been sequenced. The gene, designated G1207R, is located in the central region of the viral genome, and encodes a protein of 1207 amino acids (aa) with a predicted M(r) of 139,835. The gene is transcribed at both early and late stages of infection into a 4.1-kb RNA. Transcription is initiated at tsp, 8 nucleotides (nt) upstream from the start codon. Open reading frame (ORF) G1207R contains four direct repeats in tandem close to the 3'-end. Each repeat consists of 12 nt, coding for the reiterated sequence, K/NPAG. The deduced aa sequence of G1207R shows significant similarity with DNA polymerases from cellular and viral origin, belonging to the alpha-like family of DNA polymerases. In particular, the G1207R protein presents a colinear arrangement of all the 3'-->5' exonuclease and polymerization highly conserved aa regions characteristic of this group of DNA-dependent DNA polymerases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • African Swine Fever Virus / enzymology
  • African Swine Fever Virus / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Conserved Sequence
  • DNA, Viral
  • DNA-Directed DNA Polymerase / genetics*
  • Genes, Viral
  • Molecular Sequence Data
  • Open Reading Frames
  • Sequence Analysis, DNA*
  • Sequence Homology, Amino Acid
  • Transcription, Genetic*


  • DNA, Viral
  • DNA-Directed DNA Polymerase

Associated data

  • GENBANK/L19042
  • GENBANK/L24529
  • GENBANK/U03993
  • GENBANK/U04610
  • GENBANK/X71402
  • GENBANK/X72987
  • GENBANK/X72988
  • GENBANK/X72989
  • GENBANK/Z15047
  • GENBANK/Z15048