Genomic characterization of the human DNA excision repair-controlling gene XPAC

Gene. 1993 Dec 22;136(1-2):345-8. doi: 10.1016/0378-1119(93)90493-m.

Abstract

We have characterized the human DNA excision repair gene, XPAC (xeroderma pigmentosum group A complementing). This gene of approximately 25 kb consists of six exons. The 5'-flanking region of the gene has a CAAT box, but no TATA box. The region upstream from the coding sequence of exon 1 is G + C rich (73%), and has a GC box. Transcriptional mapping analysis suggested that there is one major transcription start point (tsp). The presence of two polyadenylation signals suggests that the two XPAC mRNAs with different 3' untranslated regions in normal human cells are due to alternative polyadenylations. The promoter activity, measured by transient expression of the cat gene with the 5' flanking regions, indicated the presence of a functional promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Repair / genetics*
  • DNA, Complementary
  • DNA-Binding Proteins / genetics*
  • Exons
  • Humans
  • Introns
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Transcription, Genetic
  • Xeroderma Pigmentosum Group A Protein

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • XPA protein, human
  • Xeroderma Pigmentosum Group A Protein
  • Xpa protein, mouse

Associated data

  • GENBANK/D14533