New vectors for direct cloning of PCR products

J Cha; W Bishai; S Chandrasegaran

doi:10.1016/0378-1119(93)90498-r

New vectors for direct cloning of PCR products

Gene. 1993 Dec 22;136(1-2):369-70. doi: 10.1016/0378-1119(93)90498-r.

Authors

J Cha¹, W Bishai, S Chandrasegaran

Affiliation

¹ Department of Environmental Health Sciences, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205.

PMID: 8294034
DOI: 10.1016/0378-1119(93)90498-r

Abstract

We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Bacteriophage M13 / genetics
Base Sequence
Cloning, Molecular / methods*
DNA
Genetic Vectors*
Molecular Sequence Data
Polymerase Chain Reaction*

Substances

DNA

Abstract

Publication types

MeSH terms

Substances

Grants and funding