A negatively light-regulated gene from Arabidopsis thaliana encodes a protein showing high similarity to blue copper-binding proteins

Gene. 1993 Dec 22;136(1-2):79-85. doi: 10.1016/0378-1119(93)90450-h.


A negatively photo-regulated gene (bcb) has been isolated by means of differential hybridization of a genomic library of Arabidopsis thaliana. In mature plants, a 20-fold increase in the amount of steady-state bcb mRNA can be detected upon 48 h of dark adaption. The expression level of the gene is also dependent upon the developmental stage of the plant. The 21.5-kDa gene product (BCB) shows extensive similarity with blue Cu(2+)-binding proteins such as plastocyanin and stellacyanin. The protein abundance increases only twofold upon dark adaption, which implies the presence of post-transcriptional control. The isolation of a novel negatively photoregulated gene allows us to investigate the complex expression profile of genes responding to the absence of light.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Azurin / analogs & derivatives*
  • Azurin / genetics
  • Base Sequence
  • Carrier Proteins / genetics*
  • Copper*
  • DNA, Complementary
  • Gene Expression Regulation* / radiation effects
  • Light*
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Plant Proteins / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid


  • Arabidopsis Proteins
  • BCB protein, Arabidopsis
  • Carrier Proteins
  • DNA, Complementary
  • Membrane Proteins
  • Plant Proteins
  • copper-binding protein
  • cupredoxin
  • Azurin
  • Copper

Associated data

  • GENBANK/L11589
  • GENBANK/L11590
  • GENBANK/L11591
  • GENBANK/L11592
  • GENBANK/L11593
  • GENBANK/L11594
  • GENBANK/L24529
  • GENBANK/Z15047
  • GENBANK/Z15048
  • GENBANK/Z15058