Anti-neutrophil cytoplasmic antibodies in giant cell arteritis and polymyalgia rheumatica

Adv Exp Med Biol. 1993:336:363-6. doi: 10.1007/978-1-4757-9182-2_61.

Abstract

Antineutrophil cytoplasmic antibodies (ANCA) were demonstrated in all sera from 11 patients with active giant cell arteritis (GCA) using indirect immunofluorescence on 9% paraformaldehyde(PF)-fixed neutrophils according to Pryzwanski (median titer 1:256, range 1:64 to 1:512). After treatment during inactive disease titers decreased in all sera. Eight out of 9 sera from patients with active polymyalgia rheumatica (PMR) produced a cytoplasmic staining on Pryzwanski-fixed neutrophils in low titers (median titer 1:16, range 0 to 1:32), as did 8 out of 25 sera from healthy blood donors. None of the sera were positive for antibodies to defined antigens, i.e. proteinase-3, human leucocyte elastase, myeloperoxidase and lactoferrin as detected by ELISA. GCA seems to be associated with ANCA of as yet unknown specificity.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Antibodies, Antineutrophil Cytoplasmic
  • Arteritis / immunology*
  • Autoantibodies / blood*
  • Female
  • Fluorescent Antibody Technique
  • Humans
  • Immunoglobulin G / blood*
  • Male
  • Middle Aged
  • Polymyalgia Rheumatica / immunology*

Substances

  • Antibodies, Antineutrophil Cytoplasmic
  • Autoantibodies
  • Immunoglobulin G