A centrifugal separation procedure detects moderate affinity cGMP binding sites in membrane-associated proteins and permeabilized cells

Anal Biochem. 1993 Nov 15;215(1):159-61. doi: 10.1006/abio.1993.1570.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anura
  • Binding Sites
  • Carrier Proteins / isolation & purification*
  • Carrier Proteins / metabolism
  • Cell Membrane Permeability
  • Cell Separation / methods
  • Centrifugation
  • Cyclic GMP / isolation & purification
  • Cyclic GMP / metabolism*
  • Intracellular Signaling Peptides and Proteins*
  • Kinetics
  • Membrane Proteins / isolation & purification*
  • Rhodopsin / isolation & purification
  • Rod Cell Outer Segment / chemistry

Substances

  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • cyclic GMP-binding protein
  • Rhodopsin
  • Cyclic GMP