Localization of osteogenic protein-1 (bone morphogenetic protein-7) during human embryonic development: high affinity binding to basement membranes

Biochem Biophys Res Commun. 1994 Jan 28;198(2):693-700. doi: 10.1006/bbrc.1994.1100.


Osteogenic protein-1 (OP-1) is a member of the bone morphogenetic protein subfamily of the transforming growth factor-beta (TGF-beta) superfamily. Since members of the TGF-beta superfamily have a role in tissue development the distribution of OP-1 in developing human embryos (gestational age 5-14 weeks) was examined by immunohistochemical methods. Positive staining for OP-1 was observed in: sclerotome, hypertrophied chondrocytes, osteoblasts, periosteum, epithelial cells of the adrenal "provisional cortex" and the convoluted tubules of developing kidneys. In the developing lungs, pancreas and skin, OP-1 was localized in basement membranes underlying the epithelium. In vitro binding studies of 125I-OP-1 to various extracellular matrix components revealed high affinity of OP-1 for type IV collagen and less for heparin, collagen types I and VI. Present findings suggest that, in addition to bone formation, OP-1 could have other important regulatory roles in human embryogenesis with high binding affinity to a basement membrane component.

MeSH terms

  • Adrenal Glands / anatomy & histology
  • Adrenal Glands / chemistry
  • Basement Membrane / chemistry*
  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins*
  • Bone and Bones / anatomy & histology
  • Bone and Bones / chemistry
  • Cartilage / anatomy & histology
  • Cartilage / chemistry
  • Embryo, Mammalian / anatomy & histology
  • Embryo, Mammalian / chemistry*
  • Extracellular Matrix / chemistry
  • Extracellular Matrix / metabolism
  • Humans
  • Kidney / anatomy & histology
  • Kidney / chemistry
  • Proteins / isolation & purification*
  • Proteins / metabolism
  • Transforming Growth Factor beta*


  • BMP7 protein, human
  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins
  • Proteins
  • Transforming Growth Factor beta