Minute virus of mice-induced modification of the murine DNA polymerase alpha-primase complex permits the salt-induced dissociation of 12S DNA primase and 10S DNA polymerase alpha components

Biochem Mol Biol Int. 1993 Nov;31(4):599-611.


DNA polymerase alpha-primase complexes in extracts of MVM-infected murine cells were dissociated in the presence of 0.3M KCl to generate a 12S DNA primase and a 10S DNA polymerase alpha that were readily separated by sedimentation in glycerol gradients. A 12S DNA polymerase alpha-primase complex refractory to dissociation in 0.3M KCl was identified in extracts of MVM-infected HeLa cells. In extracts of mock-infected murine and HeLa cells DNA primase and DNA polymerase alpha were not dissociated from each other in 0.3M KCl but remained in a stable complex that sedimented at 10S. We propose that a novel 12S DNA polymerase alpha-primase complex prone to disruption by salt is induced by MVM infection and that the DNA primase component of the complex is modified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Ehrlich Tumor / enzymology*
  • Cell Line, Transformed
  • Cell Transformation, Viral / physiology
  • Cells, Cultured
  • Cricetinae
  • Cricetulus
  • DNA Polymerase II / isolation & purification*
  • DNA Primase
  • DNA, Viral / biosynthesis
  • HeLa Cells
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Minute Virus of Mice / enzymology*
  • RNA Nucleotidyltransferases / isolation & purification*
  • RNA Nucleotidyltransferases / metabolism*
  • Salts


  • DNA, Viral
  • Salts
  • DNA Primase
  • RNA Nucleotidyltransferases
  • DNA Polymerase II