Purification and characterisation of the restriction endonuclease ItaI from Ilyobacter tartaricus recognizing 5'-GC decreases NGC-3'

P Städtler; R P von Strandmann; T Walter; B Frey; H Auer; W Hengstenberg; G Schmitz

doi:10.1016/0378-1119(93)90033-y

Purification and characterisation of the restriction endonuclease ItaI from Ilyobacter tartaricus recognizing 5'-GC decreases NGC-3'

Gene. 1993 Dec 31;137(2):347-8. doi: 10.1016/0378-1119(93)90033-y.

Authors

P Städtler¹, R P von Strandmann, T Walter, B Frey, H Auer, W Hengstenberg, G Schmitz

Affiliation

¹ Department of Microbiology, Ruhr-Universität Bochum, Germany.

PMID: 8299969
DOI: 10.1016/0378-1119(93)90033-y

Abstract

ItaI, an isoschizomer of the subclass IIW [Kessler and Manta, Gene 92 (1990) 1-248] restriction endonuclease (ENase), Fnu4HI [Leung et al., Nucleic Acids Res. 6 (1979) 17-25], has been isolated from Ilyobacter tartaricus. The ENase has the five-base palindromic recognition sequence, 5'GC decreases NGC-3'. It cleaves behind the second nucleotide and produces a one-nt 5' overhang.

MeSH terms

Deoxyribonucleases, Type II Site-Specific / isolation & purification
Deoxyribonucleases, Type II Site-Specific / metabolism*
Gram-Negative Anaerobic Bacteria / enzymology*
Repetitive Sequences, Nucleic Acid
Substrate Specificity

Substances

endodeoxyribonuclease Fnu4HI
Deoxyribonucleases, Type II Site-Specific