Long-term viral enhancement of lung response to Saccharopolyspora rectivirgula

Am J Respir Crit Care Med. 1994 Feb;149(2 Pt 1):490-4. doi: 10.1164/ajrccm.149.2.8306051.


The current study was done to look at the long-term enhancing effect of a single viral infection on repeated Saccharopolyspora rectivirgula (SR) antigenic challenges in mice and at inflammatory cytokines in this enhancement. Four groups of C57BI/6 mice were studied: Group 1 received intranasal instillations of saline, 3 days per week; Group 2, intranasal instillations of saline plus one intranasal instillation of 80 hemagglutination units (HAU) of Sendai virus after 3 wk of saline; Group 3, instillations of SR, 3 days per week; and Group 4, instillations of SR, 3 days per week, plus one instillation of Sendai virus after 3 wk of SR. Bronchoalveolar lavages (BAL) were performed 15 and 30 wk after the virus inoculation in the appropriate groups. Each time, a two- to threefold increase in BAL cell counts was obtained from virus-infected animals challenged with SR compared with animals that received the SR alone. Animals infected with virus only showed values similar to those of control animals. A higher percentage of large foamy multinucleated cells were found in the BAL from the SR+Sendai group than the SR alone group (7.92 +/- 0.730% compared with 1.8 +/- 0.296%). These cells were not seen in the other groups. BAL levels of TNF-alpha and IL-1 alpha at 15 wk were much higher in SR+Sendai-treated (1,439 +/- 268 and 96 +/- 9 pg/ml, respectively) than SR alone-treated animals (361 +/- 100 and 23 +/- 4 pg/ml). BAL fluid of control animals and Sendai alone animals contained 64 +/- 24 and 65 +/- 15 pg/ml of TNF-alpha, but no IL-1 alpha was detected.(ABSTRACT TRUNCATED AT 250 WORDS)

MeSH terms

  • Animals
  • Antigens, Bacterial / immunology*
  • Bronchoalveolar Lavage Fluid / cytology
  • Farmer's Lung / immunology
  • Farmer's Lung / microbiology*
  • Interleukin-1 / biosynthesis
  • Lung / immunology*
  • Lung / microbiology
  • Lung / pathology
  • Mice
  • Mice, Inbred C57BL
  • Parainfluenza Virus 1, Human / immunology*
  • Paramyxoviridae Infections / immunology*
  • Saccharopolyspora / immunology*
  • Specific Pathogen-Free Organisms
  • Time Factors
  • Tumor Necrosis Factor-alpha / biosynthesis


  • Antigens, Bacterial
  • Interleukin-1
  • Tumor Necrosis Factor-alpha