An algorithm accounting for plating efficiency in estimating spontaneous mutation rates

Comput Biol Med. 1993 Nov;23(6):455-61. doi: 10.1016/0010-4825(93)90093-g.


An algorithm is described for calculating the probability distribution of the number of mutant colonies arising in a Luria-Delbrück fluctuation experiment subject to a plating efficiency of less than 100%. A table is constructed to facilitate the estimation of spontaneous mutation rates taking into account the plating efficiency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Cells, Cultured
  • Clone Cells
  • Models, Genetic*
  • Mutation / physiology*
  • Probability
  • Selection, Genetic