The role of trehalose synthesis for the acquisition of thermotolerance in yeast. I. Genetic evidence that trehalose is a thermoprotectant

Eur J Biochem. 1994 Jan 15;219(1-2):179-86. doi: 10.1111/j.1432-1033.1994.tb19928.x.


In the yeast Saccharomyces cerevisiae, accumulation of the non-reducing disaccharide trehalose is triggered by various stimuli that activate the heat-schock response. Several studies have shown a close correlation between trehalose levels and tolerance to heat stress, suggesting that trehalose may be a protectant which contributes to thermotolerance. In this study, we have examined mutants defective in genes coding for key enzymes involved in trehalose metabolism with respect to the heat-induced and stationary-phase-induced accumulation of trehalose and the acquisition of thermotolerance. Inactivation of either TPS1 or TPS2, encoding subunits of the trehalose-6-phosphate synthase/phosphatase complex, caused an inability to accumulate trehalose upon a mild heat-shock or upon initiation of the stationary phase and significantly reduced the levels of heat-induced and stationary-phase-induced thermotolerance. Deletion of NTH1, the gene coding for the neutral trehalase, resulted in a defect in trehalose mobilization during recovery from a heat shock which was paralleled by an abnormally slow decrease of thermotolerance. Our results provide strong genetic evidence that heat-induced synthesis of trehalose is an important factor for thermotolerance induction. In an accompanying study [Hottiger, T., De Virgilio, C., Hall, M. N., Boller, T. & Wiemken, A. (1993) Eur. J. Biochem. 219, 187-193], we present evidence that the function of heat-induced trehalose accumulation may be to increase the thermal stability of proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acclimatization / genetics
  • Genes, Fungal*
  • Glucosyltransferases / biosynthesis
  • Glucosyltransferases / genetics
  • Hot Temperature
  • Kinetics
  • Macromolecular Substances
  • Multienzyme Complexes / biosynthesis
  • Multienzyme Complexes / genetics
  • Phosphoric Monoester Hydrolases / biosynthesis
  • Phosphoric Monoester Hydrolases / genetics
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae / physiology*
  • Species Specificity
  • Sugar Phosphates / biosynthesis
  • Time Factors
  • Trehalase / biosynthesis
  • Trehalase / genetics
  • Trehalose / analogs & derivatives
  • Trehalose / biosynthesis*


  • Macromolecular Substances
  • Multienzyme Complexes
  • Sugar Phosphates
  • trehalose-6-phosphate
  • Trehalose
  • Glucosyltransferases
  • trehalose-6-phosphate synthase-phosphatase complex
  • Phosphoric Monoester Hydrolases
  • Trehalase