Hepatocyte growth factor elevates the activity levels of glycolipid sulfotransferases in renal cell carcinoma cells

Eur J Biochem. 1994 Jan 15;219(1-2):407-13. doi: 10.1111/j.1432-1033.1994.tb19953.x.


Accumulation of sulfoglycolipids associated with markedly elevated activity levels of glycolipid sulfotransferases has previously been demonstrated in the human renal cell carcinoma cell line, SMKT-R3. To elucidate the regulatory mechanisms of sulfoglycolipid synthesis in SMKT-R3 cells, the effects of various growth factors on the metabolic enzymes of sulfoglycolipids were investigated. Hepatocyte growth factor (HGF) significantly increased the activity levels of the sulfotransferases in a dose-dependent manner, but did not change that of arylsulfatase A, which hydrolyzes sulfoglycolipids. Scatchard analysis of 125I-HGF binding to SMKT-R3 cells indicated that the cells expressed high-affinity receptors for HGF with a Kd of 36 pM and 750 sites/cell. Furthermore, metabolic labeling with [35S]sulfate revealed that the addition of HGF to the culture medium of the cells resulted in an increment of sulfoglycolipid synthesis. Therefore, these observations suggest that HGF can function as a regulatory factor in sulfoglycolipid synthesis through the modulation of the sulfotransferase activity levels in renal cell carcinoma cells. In addition, HGF stimulated the proliferation and motility of SMKT-R3 cells, suggesting that HGF has multiple biological activities in renal cell carcinoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Carcinoma, Renal Cell / enzymology*
  • Cell Division / drug effects
  • Cell Line
  • Cell Movement / drug effects
  • Cricetinae
  • Cycloheximide / pharmacology
  • DNA, Neoplasm / biosynthesis
  • DNA, Neoplasm / drug effects
  • Dose-Response Relationship, Drug
  • Glycolipids / biosynthesis
  • Growth Substances / pharmacology
  • Hepatocyte Growth Factor / biosynthesis
  • Hepatocyte Growth Factor / isolation & purification
  • Hepatocyte Growth Factor / pharmacology*
  • Humans
  • Immune Sera / pharmacology
  • Kidney Neoplasms / enzymology*
  • Kinetics
  • Mice
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / pharmacology
  • Sulfotransferases / metabolism*
  • Transfection
  • Tumor Cells, Cultured


  • DNA, Neoplasm
  • Glycolipids
  • Growth Substances
  • Immune Sera
  • Recombinant Proteins
  • Hepatocyte Growth Factor
  • Cycloheximide
  • Sulfotransferases
  • galactosylceramide sulfotransferase