Chromosomal localization of the mouse titin gene and its relation to "muscular dystrophy with myositis" and nebulin genes on chromosome 2

Genomics. 1993 Dec;18(3):559-61. doi: 10.1016/s0888-7543(05)80356-8.


In the mouse, the genes for the structural components of the myofibril titin and nebulin, Ttn and Neb, map to proximal Chr 2, as does the gene for a muscle disease, "muscular dystrophy with myositis," mdm. To facilitate the evaluation of Ttn and Neb as possible candidates for mdm, we have determined their relative map positions, using a Mus spretus/Mus musculus interspecific backcross. The gene order (distances in cM) cenVim-16.9 +/- 4.7-Neb-7.6 +/- 3.0-Ttn, Acra-18.0 +/- 4.9-Pax-6-17.7 +/- 4.9-a ... has been determined. Considering the standard deviations, Neb, Ttn, and Acra could colocalize with mdm. Using Ttn and Neb probes, DNAs from mdm/mdm and mdm/+ mice were tested for restriction fragment variants in comparison to the M. musculus wildtype. No variants have been found with 11 restriction nucleases. Our data corroborate a conserved synteny comprising genes NEB, TTN, CHRNA1 on human Chr 2q.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • Connectin
  • Conserved Sequence
  • DNA Primers
  • Haplotypes
  • Humans
  • Membrane Proteins / genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains / genetics*
  • Molecular Sequence Data
  • Muridae / genetics
  • Muscle Proteins / genetics*
  • Muscular Dystrophy, Animal / complications
  • Muscular Dystrophy, Animal / genetics*
  • Myositis / complications
  • Myositis / genetics*
  • Polymerase Chain Reaction
  • Protein Kinases*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid


  • Connectin
  • DNA Primers
  • Membrane Proteins
  • Muscle Proteins
  • TTN protein, human
  • nebulin
  • Protein Kinases

Associated data

  • GENBANK/X64700
  • GENBANK/X70032