Identification of radiolabeled metabolites of nicotine in rat bile. Synthesis of S-(-)-nicotine N-glucuronide and direct separation of nicotine-derived conjugates using high-performance liquid chromatography

J Chromatogr. 1993 Nov 17;621(1):49-53.


Four metabolites of nicotine, including two glucuronides, have been separated by high-performance liquid chromatography. This separation was applied to identification of biliary metabolites of radiolabeled nicotine by radiometric detection. S-(-)-Nicotine N-glucuronide was synthesized and used as a standard in method development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bile / chemistry*
  • Chromatography, High Pressure Liquid / methods*
  • Glucuronates / chemical synthesis*
  • Magnetic Resonance Spectroscopy
  • Male
  • Mass Spectrometry
  • Nicotine / analogs & derivatives*
  • Nicotine / analysis*
  • Nicotine / chemical synthesis
  • Nicotine / metabolism
  • Radiometry
  • Rats
  • Rats, Sprague-Dawley


  • Glucuronates
  • nicotine N-glucuronide
  • Nicotine