Thick-section fluorescence in situ hybridization on formalin-fixed, paraffin-embedded archival tissue provides a histogenetic profile

Am J Pathol. 1994 Feb;144(2):237-43.


Fluorescence in situ hybridization has become a major tool for analysis of gene and chromosome copy number in normal and malignant tissue. The technique has been applied widely to fresh tissue and dispersed formalin-fixed, paraffin-embedded archival tissue, but its use on sections of archival tissue has largely been limited to sections < 6 mu thick. This does not provide intact, uncut nuclei for accurate analysis of gene or chromosome copy number. We report here a method of hybridization to sections > 20 microns thick that overcomes these difficulties. Key developments were the use of DNA probes directly labeled with fluorochromes and optical sectioning using laser-scanning confocal microscopy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosomes, Human, Pair 1 / metabolism*
  • DNA Probes
  • Deoxyuracil Nucleotides
  • Fluoresceins
  • Formaldehyde
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Melanoma / metabolism*
  • Microscopy
  • Microtomy
  • Paraffin Embedding*
  • Skin Neoplasms / metabolism*
  • Tissue Fixation*


  • DNA Probes
  • Deoxyuracil Nucleotides
  • Fluoresceins
  • fluorescein-11-deoxyuridine triphosphate
  • Formaldehyde