FcR gamma chain deletion results in pleiotrophic effector cell defects

Cell. 1994 Feb 11;76(3):519-29. doi: 10.1016/0092-8674(94)90115-5.

Abstract

The gamma subunit of immunoglobulin Fc receptors is an essential component of the high-affinity receptor for IgG (Fc gamma RIII) and is associated with the high-affinity receptor for IgG (Fc gamma RI) and the T cell receptor-CD3 complex. It is required for both receptor assembly and signal transduction. Targeted disruption of this subunit results in immunocompromised mice. Activated macrophages from gamma chain-deficient mice unexpectedly lack the ability to phagocytose antibody-coated particles, despite normal binding. Defects in NK cell-mediated antibody-dependent cytotoxicity and mast cell-mediated allergic responses are evident in these animals, establishing the indispensable role of FcRs in these responses. However, loss of gamma chain does not appear to perturb T cell development, since both thymic and peripheral T cell populations appear normal. These mice thus represent an important tool for evaluating the role of these receptors in humoral and cellular immune responses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cells, Cultured
  • Chimera
  • Cytotoxicity, Immunologic*
  • DNA / genetics
  • DNA / isolation & purification
  • DNA Primers
  • Female
  • Flow Cytometry
  • Gene Deletion*
  • Genotype
  • Interleukin-4 / biosynthesis
  • Macromolecular Substances
  • Macrophages, Peritoneal / immunology*
  • Male
  • Mast Cells / immunology*
  • Mast Cells / physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Molecular Sequence Data
  • Passive Cutaneous Anaphylaxis
  • Phagocytosis
  • Polymerase Chain Reaction
  • Prostaglandin D2 / metabolism
  • RNA, Messenger / biosynthesis
  • Receptors, IgE / genetics
  • Receptors, IgE / physiology*
  • Receptors, IgG / genetics
  • Receptors, IgG / physiology*
  • Spleen / immunology
  • Stem Cells
  • T-Lymphocytes / immunology*

Substances

  • DNA Primers
  • Macromolecular Substances
  • RNA, Messenger
  • Receptors, IgE
  • Receptors, IgG
  • Interleukin-4
  • DNA
  • Prostaglandin D2