cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis

EMBO J. 1994 Jan 15;13(2):425-34.

Abstract

We have used an immunoprecipitation-PCR cycle to isolate physically genomic DNA sequences that are bound by the fission yeast cdc10 gene product in an attempt to identify novel target genes. An essential gene, cdt1, has been isolated whose expression is cell cycle regulated in a cdc10 dependent manner. The cdt1 promoter contains a recognition site for a sequence specific DNA binding factor. The cdc10 gene product is a component of this factor. Ectopic expression of cdt1 can complement a temperature sensitive mutation of cdc10 at semipermissive temperature. Cells carrying a null allele of cdt1 are defective in DNA replication but initiate mitotic events, suggesting that cdt1 is essential for the normal dependency relationship of S-phase and mitosis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • Cell Cycle Proteins / metabolism*
  • Cloning, Molecular
  • DNA Replication*
  • DNA, Fungal
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / metabolism*
  • Genetic Complementation Test
  • Mitosis / genetics*
  • Molecular Sequence Data
  • Phenotype
  • Promoter Regions, Genetic
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces pombe Proteins
  • Temperature
  • Transcription Factors / metabolism*

Substances

  • Cell Cycle Proteins
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • cdc10 protein, S pombe
  • cdt1 protein, S pombe

Associated data

  • GENBANK/Z27248