Identification of a novel vertebrate cyclin: cyclin B3 shares properties with both A- and B-type cyclins

EMBO J. 1994 Feb 1;13(3):595-605.

Abstract

Cyclins play a key role in controlling progression through the cell cycle. They act as regulatory subunits of p34cdc2/CDC28 and related cyclin-dependent protein kinases (cdks). In vertebrates, cyclins B1 and B2 function during M phase, whereas cyclin A is required for S phase as well as the G2 to M phase transition. Here, we describe the identification and characterization of a novel vertebrate cyclin, termed cyclin B3. The assignment of this cyclin to the B-type subfamily is based on its cDNA-derived sequence and its pattern of expression in synchronized cells, both suggesting a distant relationship to other B-type cyclins. Interestingly, however, cyclin B3 also displays properties that resemble those of A- rather than B-type cyclins. Specifically, cyclin B3 localizes to the cell nucleus throughout the cell cycle, and is able to associate in vivo with at least two kinase subunits, p34cdc2 and p33cdk2. Furthermore, deletion of 26 amino acids from the C-terminus of cyclin B3 impairs both its interaction with kinase catalytic subunits and its nuclear localization, reminiscent of recent results obtained with cyclin A. Based on these observations, we conclude that cyclin B3 may share functional properties with both A- and B-type cyclins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CDC2 Protein Kinase / metabolism
  • CDC2-CDC28 Kinases*
  • Cell Line
  • Chick Embryo
  • Cloning, Molecular
  • Cyclin B*
  • Cyclin-Dependent Kinase 2
  • Cyclin-Dependent Kinases*
  • Cyclins / analysis*
  • Cyclins / genetics
  • DNA
  • HeLa Cells
  • Humans
  • Immunohistochemistry
  • Molecular Sequence Data
  • Protein Kinases / metabolism
  • Protein-Serine-Threonine Kinases*
  • Sequence Homology, Amino Acid
  • Vertebrates

Substances

  • CCNB3 protein, human
  • Cyclin B
  • Cyclins
  • DNA
  • Protein Kinases
  • Protein-Serine-Threonine Kinases
  • CDC2 Protein Kinase
  • CDC2-CDC28 Kinases
  • CDK2 protein, human
  • Cyclin-Dependent Kinase 2
  • Cyclin-Dependent Kinases

Associated data

  • GENBANK/X75757