Abstract
The genomic structure of the POU domain containing oct-1 gene (OTF1 locus) coding region has been determined using human DNA recombinant bacteriophage and a yeast artificial chromosome clone. The gene is encoded by 16 exons spanning over 150 kb, and the Oct-1 protein reading frame has been extended to 766 amino acids. The exonic structure has been compared to the mouse Oct-2 protein and reveals a conservation of exon-intron boundaries as well as protein sequence similarity. To provide insight into Oct-1 control of transcriptional regulation during the cell-cycle the expression of the oct-1 gene was examined during cellular DNA replication and shows that the steady-state level of the oct-1 mRNA is not S-phase regulated.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Line
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Chromosomes, Fungal
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Chromosomes, Human, Pair 1*
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Cloning, Molecular
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DNA, Recombinant
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DNA-Binding Proteins / genetics*
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Exons
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Gene Expression Regulation
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Gene Library
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Genes*
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Genome, Human
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Histones / biosynthesis
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Histones / genetics
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Host Cell Factor C1
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Humans
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Molecular Sequence Data
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Multigene Family
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Octamer Transcription Factor-1
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Octamer Transcription Factor-2
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Open Reading Frames
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RNA, Messenger / genetics
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S Phase
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / genetics*
Substances
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DNA, Recombinant
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DNA-Binding Proteins
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HCFC1 protein, human
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Histones
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Host Cell Factor C1
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Octamer Transcription Factor-1
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Octamer Transcription Factor-2
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POU2F1 protein, human
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POU2F2 protein, human
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Pou2f2 protein, mouse
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RNA, Messenger
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Transcription Factors