[Isolation of protein phosphokinase from a complex form of DNA polymerase alpha from rat liver]

Mol Biol (Mosk). 1993 May-Jun;27(3):531-7.
[Article in Russian]


Protein kinase activity was revealed in complex forms of rat liver DNA polymerase alpha containing 3'-5'-exonuclease, primase, helicase, DNA ligase. Protein kinase (mol. mass about 200 kDa) has been partially purified from a specimen of high molecular mass DNA polymerase alpha of nuclear membrane of regenerating liver. The protein kinase activity of the complex form of DNA polymerase alpha was maximal in the cytosol in normal rat liver cells and in the nuclear membrane in dividing cells (40 h after partial hepatectomy). The main phosphokinase properties of this enzyme were determined.

MeSH terms

  • Animals
  • Cell Nucleus / enzymology
  • Chromatography, Liquid
  • DNA Polymerase II / metabolism*
  • Intracellular Membranes / enzymology
  • Liver / enzymology*
  • Liver / physiology
  • Liver Regeneration
  • Male
  • Protein Kinases / isolation & purification*
  • Protein Kinases / metabolism
  • Rats


  • Protein Kinases
  • DNA Polymerase II