The present study, aimed at investigating effects of film-induced amusement on saliva cortisol levels, was performed on 52 healthy male volunteers, aged 19-31 years. They were assigned to an amusement or a control condition. Saliva cortisol and affective film ratings were the dependent variables. During amusement 50% of the subjects showed changes in saliva cortisol correlated positively with ratings of funniness. Thus, cortisol secretion may be linked to emotional arousal, regardless of the emotional valence.