Purification and substrate specificity of beta-xylosidase from sycamore cell (Acer pseudoplatanus L.): application for structural analysis of xylose-containing N-linked oligosaccharides

Anal Biochem. 1993 Jun;211(2):205-9. doi: 10.1006/abio.1993.1258.


A beta-xylosidase was purified 51-fold from culture medium of sycamore (Acer pseudoplatanus L.) cells using p-nitrophenyl beta-D-xylopyranoside as a substrate. This enzyme can remove a xylose residue from asparagine-linked oligosaccharides, derivatized with 2-aminopyridine. A pentasaccharide, Xy1 beta 2Man beta 4GlcNAc beta 4(Fuc-alpha 3)GlcNAc was the favorite substrate in N-linked oligosaccharides, but a xylose residue in Xy1 beta 2(Man-alpha 3)Man beta sequence could not be removed by the enzyme. We also propose an efficient method for detection of xylose residue in N-linked oligosaccharides by a combination of the two-dimensional sugar mapping technique and the xylosidase digestion.

MeSH terms

  • Carbohydrate Sequence
  • Cells, Cultured
  • Kinetics
  • Molecular Sequence Data
  • Molecular Structure
  • Oligosaccharides / analysis*
  • Oligosaccharides / chemistry
  • Oligosaccharides / metabolism
  • Plant Cells
  • Plant Proteins / isolation & purification*
  • Plant Proteins / metabolism*
  • Plants / enzymology*
  • Substrate Specificity
  • Xylose / analysis*
  • Xylose / chemistry
  • Xylose / metabolism
  • Xylosidases / isolation & purification*
  • Xylosidases / metabolism*


  • Oligosaccharides
  • Plant Proteins
  • Xylose
  • Xylosidases
  • exo-1,4-beta-D-xylosidase