Isolation and sequence of HSP30, a yeast heat-shock gene coding for a hydrophobic membrane protein

Curr Genet. May-Jun 1993;23(5-6):435-42. doi: 10.1007/BF00312631.

Abstract

Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously known protein have been noted. The protein is very hydrophobic and is predicted to have a membraneous localisation. In agreement with this hypothesis, the analysis of membrane proteins from stationary-phase cells showed that a strain carrying this gene on a multicopy vector overproduces a protein of 30 kDa. This protein was recognized by antibodies directed against the N-terminal portion of the gene product. Considering its induction in response to heat shock and the apparent molecular weight of its product, this gene was designated HSP30.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • Codon
  • DNA, Fungal / isolation & purification
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal
  • Genes, Fungal*
  • HSP30 Heat-Shock Proteins
  • Heat-Shock Proteins / genetics*
  • Heat-Shock Proteins / metabolism
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins

Substances

  • Codon
  • DNA, Fungal
  • Fungal Proteins
  • HSP30 Heat-Shock Proteins
  • HSP30 protein, S cerevisiae
  • Heat-Shock Proteins
  • Membrane Proteins
  • Saccharomyces cerevisiae Proteins

Associated data

  • GENBANK/M93123
  • GENBANK/X68488
  • GENBANK/X68489
  • GENBANK/X68490
  • GENBANK/X71675
  • GENBANK/X71676
  • GENBANK/X71677
  • GENBANK/X71678
  • GENBANK/X71679
  • GENBANK/X71680