Identification, characterization and sequence of Candida albicans repetitive DNAs Rel-1 and Rel-2

Curr Genet. May-Jun 1993;23(5-6):455-62. doi: 10.1007/BF00312634.

Abstract

Two moderately repetitive DNA elements, Rel-1 and Rel-2, were identified in a screen for clones that hybridized to a Candida albicans minichromosome. Rel-1, a 223-bp sequence, is C. albicans-specific. The 2789-bp Rel-2 sequence hybridizes weakly to C. stellatoidia DNA but not to DNA from several other yeast species. Genomic Southern-blot analysis indicated that Rel-1 and Rel-2 are often closely associated in the genome, suggesting that they may be subsequences of a larger repetitive element. Small subrepeats are located in the nucleotide sequence of both clones. Hybridization demonstrated that Rel-2 contains both repetitive and unique DNA sequences. The repetitive DNA is present on most, and perhaps all, C. albicans chromosomes. The unique sequence maps to chromosome 7; however, in some strains, it is also present on additional chromosomes.

MeSH terms

  • Base Sequence
  • Candida albicans / genetics*
  • Chromosomes, Fungal
  • Cloning, Molecular
  • DNA, Fungal
  • Electrophoresis, Gel, Pulsed-Field
  • Molecular Sequence Data
  • Repetitive Sequences, Nucleic Acid*
  • Restriction Mapping

Substances

  • DNA, Fungal

Associated data

  • GENBANK/L11896
  • GENBANK/L11897
  • GENBANK/X68489
  • GENBANK/X68490
  • GENBANK/X71675
  • GENBANK/X71676
  • GENBANK/X71677
  • GENBANK/X71678
  • GENBANK/X71679
  • GENBANK/X71680