Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2

Curr Genet. May-Jun 1993;23(5-6):547-8. doi: 10.1007/BF00312650.

Abstract

Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Genetic Vectors*
  • Plasmids*
  • Restriction Mapping
  • Schizosaccharomyces / genetics*