The authors review the literature regarding bioptic techniques which are used in oral oncology, particularly referring to the dispute about incisional and excisional biopsy in cases of neoplastic or suspected lesions. As a matter of fact, though incisional biopsy at the oral cavity level is an easily executed technique, some clinicians found that an incision of a neoplasia could help its spreading or could help its spreading or could even increase its malignancy. Nevertheless, analyzing the literature, it can't be found, at present, the existence of data based on real clinical survey, which could prove a worse prognosis subsequent to a certain bioptic approach, even if some experimental studies on animal models, pointed out the potential danger caused by a neoplasia's incision. But, according to the authors, the oncological risk of metastasis' formation or of a local insemination is irrelevant if the operator follows the general rules of oncological surgery. To conclude, the authors present their diagnostic operative protocol, based on the principle that the diagnosis must always precede the treatment of the pathology. Therefore, when the size and site of the lesions allow it, incisional biopsy (simple or multiple) must always be chosen and, if necessary, must be preceded by the tolonium chloride stain.