Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast

Nature. 1993 Jul 8;364(6433):121-6. doi: 10.1038/364121a0.


A novel genetic selection in yeast has been used to isolate a complementary DNA for the transcriptional activator, Olf-1, which binds to the regulatory sequences of several olfactory-specific genes. The Olf-1 protein, expressed exclusively in the olfactory receptor neurons and their precursors, contains a new helix-loop-helix motif and functions as an apparent homodimer. Olf-1 may be the first member of a family of related proteins that may direct cellular differentiation in a variety of neuronal tissues.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • DNA-Binding Proteins / genetics*
  • Genetic Vectors
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Neurons, Afferent / chemistry*
  • Olfactory Mucosa / innervation
  • Olfactory Pathways / chemistry*
  • Plasmids
  • Protein Structure, Secondary
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins
  • Regulatory Sequences, Nucleic Acid
  • Saccharomyces cerevisiae / genetics
  • Sequence Homology, Amino Acid
  • Trans-Activators*
  • Transcription Factors / genetics*


  • DNA-Binding Proteins
  • Ebf1 protein, rat
  • Nerve Tissue Proteins
  • Recombinant Fusion Proteins
  • Trans-Activators
  • Transcription Factors