Pseudomonas syringae pv. syringae harpinPss: a protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plants

Cell. 1993 Jul 2;73(7):1255-66. doi: 10.1016/0092-8674(93)90354-s.

Abstract

The ability of P. syringae to elicit the hypersensitive response in nonhost plants or pathogenesis in hosts is controlled by hrp genes. The P. syringae pv. syringae 61 hrpZ gene encodes harpinPss, a 34.7 kd extracellular protein that elicits hypersensitive necrosis in tobacco and other plants. HarpinPss is heat stable, glycine rich, dissimilar in amino acid sequence to any known protein, produced only in apoplastic fluid-mimicking minimal media, and secreted in a HrpH-dependent manner. The carboxy-terminal 148 amino acid portion of harpinPss contains two directly repeated sequences of GGGLGTP and QTGT and is sufficient and necessary for elicitor activity. The necrosis elicited by harpinPss is an active response of the plant, which can be inhibited by alpha-amanitin, cycloheximide, lanthanum chloride, or sodium vanadate.

MeSH terms

  • Amanitins / pharmacology
  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins / antagonists & inhibitors
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Outer Membrane Proteins / metabolism
  • Base Sequence
  • Cycloheximide / pharmacology
  • Lanthanum / pharmacology
  • Molecular Sequence Data
  • Plants, Toxic*
  • Pseudomonas / metabolism*
  • Repetitive Sequences, Nucleic Acid
  • Tobacco / metabolism*
  • Vanadates / pharmacology

Substances

  • Amanitins
  • Bacterial Outer Membrane Proteins
  • harpin protein, Erwinia amylovora
  • lanthanum chloride
  • HrpZ protein, Pseudomonas syringae
  • Vanadates
  • Lanthanum
  • Cycloheximide

Associated data

  • GENBANK/L09219
  • GENBANK/L09220
  • GENBANK/L09221
  • GENBANK/L09222
  • GENBANK/L09223
  • GENBANK/L09224
  • GENBANK/L09225
  • GENBANK/L14775
  • GENBANK/L33709
  • GENBANK/Z22666