New simplified molecular design for functional T cell receptor

Eur J Immunol. 1993 Jul;23(7):1435-9. doi: 10.1002/eji.1830230705.

Abstract

We have produced a chimeric single-chain T cell receptor (TcR) that combines the specific antibody recognition function and TcR/CD3 signaling properties within the same polypeptide chain. This hybrid molecule consisted of a single-chain antibody combining site that was connected over a short spacer to the transmembrane and cytoplasmic region of CD3 zeta. When expressed on TcR- or TcR+ T cell hybridomas it could mediate recognition of relevant target cells and subsequent production of lymphokines; i.e. it could functionally replace the TcR/CD3 complex. Therefore, the single-chain TcR model presented here represents an interesting and useful means for the creation of T cells with new specificities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites, Antibody
  • CD3 Complex / chemistry*
  • Drug Design
  • Humans
  • Immunoglobulin Fab Fragments / chemistry
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Receptors, Antigen, T-Cell / chemistry*
  • Recombinant Fusion Proteins
  • Signal Transduction
  • Structure-Activity Relationship
  • Transfection
  • Tumor Cells, Cultured

Substances

  • CD3 Complex
  • Immunoglobulin Fab Fragments
  • Oligonucleotides
  • Receptors, Antigen, T-Cell
  • Recombinant Fusion Proteins