Robust induction of AP-1 transcription factor DNA binding activity in the hippocampus of aged rats

Neurosci Lett. 1993 Apr 30;153(2):189-91. doi: 10.1016/0304-3940(93)90319-g.

Abstract

Activation of expression of genes encoding components of AP-1 transcription factor (c-fos, c-jun and their cognates) as well as AP-1 itself has been repeatedly shown to coincide with long-term cellular responses. For the proliferating cells where functional involvement of AP-1 in physiological activity has been proved, cellular senescence as well as aging at level of the organism results in an alteration of the AP-1. The aim of the present study was to analyze whether there is an aging-related defect in the formation of AP-1 transcription factor in the brain. AP-1 has been shown previously to be inducible by several stimuli in the cells of the central nervous system, including proconvulsant treatment of pentylenetetrazole (PTZ). The present studies found that PTZ robustly induced AP-1 DNA binding activity, evaluated by the electrophoretic mobility shift assay, in hippocampi of aged (2-year-old) rats, indicating no aging-related AP-1 defect in the rat brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / metabolism*
  • Animals
  • Base Sequence
  • Brain Chemistry / drug effects
  • DNA / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression / drug effects
  • HeLa Cells
  • Hippocampus / drug effects
  • Hippocampus / metabolism*
  • Humans
  • Molecular Sequence Data
  • Pentylenetetrazole / pharmacology
  • Proto-Oncogene Proteins c-jun / metabolism*
  • Rats
  • Rats, Wistar

Substances

  • Proto-Oncogene Proteins c-jun
  • DNA
  • Pentylenetetrazole