Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase

Int J Cancer. 1977 Jan;19(1):128-35. doi: 10.1002/ijc.2910190118.


An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified. Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa-2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa-2 grows in soft agar with a colony-forming efficiency of 19%. Both MIA PaCa-2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.

MeSH terms

  • Aged
  • Aneuploidy
  • Asparaginase / pharmacology*
  • Carmustine / pharmacology
  • Cell Division / drug effects
  • Cell Line
  • Cells, Cultured
  • Cytarabine / pharmacology
  • Fibrinolysis / drug effects
  • Humans
  • Male
  • Pancreatic Neoplasms* / genetics
  • Plasminogen / biosynthesis
  • Vincristine / pharmacology


  • Cytarabine
  • Vincristine
  • Plasminogen
  • Asparaginase
  • Carmustine