A new automated method for isolation of genomic DNA from eukaryotic cells

Boll Soc Ital Biol Sper. 1993 Jan;69(1):7-11.

Abstract

In this study we determine whether the BIOMEK-1000 Laboratory Workstation (Beckman Instruments, Fullerton, CA) can be used to isolate genomic DNA from eukaryotic cells. The results obtained demonstrate that DNA isolated by BIOMEK-1000 is suitable for amplification of genomic sequences by PCR. Our data prove that automated chromatographic DNA isolation employing the BIOMEK-1000 could be used in molecular diagnosis of human pathologies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Automation
  • Chromatography, Ion Exchange / instrumentation
  • Chromatography, Ion Exchange / methods*
  • DNA / isolation & purification*
  • DNA Probes, HLA
  • DNA, Neoplasm / isolation & purification
  • Eukaryotic Cells / chemistry*
  • Humans
  • Polymerase Chain Reaction
  • Tumor Cells, Cultured

Substances

  • DNA Probes, HLA
  • DNA, Neoplasm
  • DNA