Expression of wnt10a in the central nervous system of developing zebrafish

Dev Biol. 1993 Jul;158(1):113-21. doi: 10.1006/dbio.1993.1172.

Abstract

A polymerase chain reaction-derived clone with sequence homology to partial-length shark and salamander Wnt-10a was used to screen a zebrafish cDNA library, yielding a cDNA encoding a full-length Wnt polypeptide. Transcripts encoding zebrafish wnt10a are first detected during the segmentation period of embryogenesis, at a time when there is extensive development and differentiation of the central nervous system. wnt10a transcripts are confined to differentiating brain regions, with expression first appearing diffusely in the floor of the hindbrain ventricle and proceeding anteriorly to the mid- and forebrain. Comparatively, zebrafish wnt1 transcripts are expressed in a stripe at the future midbrain-hindbrain boundary and at the future forebrain-midbrain border. With the differentiation of the neural tube into distinct regions, wnt1 signals are also detected along the dorsal midline of the midbrain, the mid- and posterior hindbrain, and the spinal cord. These patterns suggest roles for wnt10a in the regionalization of the brain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / embryology*
  • Brain Chemistry
  • Conserved Sequence
  • Gene Expression Regulation
  • Molecular Sequence Data
  • Morphogenesis
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / genetics*
  • Proto-Oncogene Proteins / biosynthesis
  • Proto-Oncogene Proteins / genetics*
  • Sequence Homology, Amino Acid
  • Wnt Proteins
  • Zebrafish / embryology*
  • Zebrafish Proteins*

Substances

  • Nerve Tissue Proteins
  • Proto-Oncogene Proteins
  • Wnt Proteins
  • Wnt10a protein, zebrafish
  • Zebrafish Proteins

Associated data

  • GENBANK/S54400
  • GENBANK/S54402
  • GENBANK/S54403
  • GENBANK/S54406
  • GENBANK/S54407
  • GENBANK/S54408
  • GENBANK/S54409
  • GENBANK/S54411
  • GENBANK/U02544
  • GENBANK/X69679