Heterogeneity of NGF-induced differentiation in PC12 cells investigated in a battery of isolated cell clones

Funct Neurol. Mar-Apr 1993;8(2):109-13.


The effect of NGF on a set of 17 PC12 cell clones was investigated in order to clarify some aspects concerning the changes in cell morphology and Ca2+ homeostasis that occur in the PC12 cell line after NGF-induced differentiation. The morphological parameters analyzed showed a high degree of heterogeneity among clones, consisting with a different expression of the high-affinity NGF receptor. The NGF effect on Ca2+ influx was investigated in a clone highly sensitive to the cholinergic agonist carbachol. Results showed an increase in Ca2+ influx through one of the carbachol-activated Ca2+ channels.

MeSH terms

  • Animals
  • Calcium / metabolism
  • Carbachol / metabolism
  • Cell Differentiation*
  • Clone Cells
  • Homeostasis
  • Nerve Growth Factors / administration & dosage
  • Nerve Growth Factors / pharmacology*
  • Neurites / drug effects*
  • PC12 Cells
  • Rats
  • Receptors, Muscarinic / drug effects


  • Nerve Growth Factors
  • Receptors, Muscarinic
  • Carbachol
  • Calcium