Deoxycytidine methylation and the origin of spontaneous transition mutations in mammalian cells

Somat Cell Mol Genet. 1993 May;19(3):275-83. doi: 10.1007/BF01233075.

Abstract

Previously we described a recurrent, site-specific G4784 --> A transition mutation affecting exon V of the Chinese hamster ovary cell RPS14 gene. Because the mutation is located within a CpG dinucleotide, we considered the possibility that deoxycytidine methylation might be responsible for the transition's unusually high frequency and site specificity. Therefore, we used a procedure based on the PCR amplification of bisulfite-modified genomic DNA to analyze the pattern of DNA cytosine methylation in exon V of the CHO cell RPS14 locus. Our data indicate that the CpG dinucleotide targeted by the transition mutation is stably methylated in CHO cell chromosomes. This finding supports the notion that deoxycytidine methylation promotes "spontaneous", site-specific transition mutations in mammalian cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells
  • Cricetinae
  • DNA Mutational Analysis
  • DNA, Single-Stranded
  • Deoxycytidine / metabolism*
  • Exons
  • Methylation
  • Molecular Sequence Data
  • Mutation*
  • Polymerase Chain Reaction

Substances

  • DNA, Single-Stranded
  • Deoxycytidine

Associated data

  • GENBANK/M35008