Recombinant interferon-gamma enhances resistance to acute disseminated Candida albicans infection in mice

J Infect Dis. 1993 Aug;168(2):436-43. doi: 10.1093/infdis/168.2.436.

Abstract

The effect of recombinant rat interferon-gamma (rIFN-gamma) on acute disseminated Candida albicans infection in mice was investigated. Outgrowth of C. albicans in kidneys, spleen, and liver of mice treated with one intravenous (iv) dose of rIFN-gamma before iv injection of 5 x 10(5) cfu of C. albicans was significantly lower than in controls over 7 days. rIFN-gamma was protective when given 1 day before, simultaneously with, or 1-3 days after infection but not when given 3 days before. In mice pretreated with hydrocortisone acetate, rIFN-gamma significantly reduced the outgrowth only when 10(3) cfu of C. albicans was injected. Injection of rIFN-gamma did not reduce the outgrowth of C. albicans in cyclophosphamide-pretreated mice and significantly increased the capacity of peripheral blood and exudate peritoneal granulocytes to kill C. albicans in vitro. Thus, rIFN-gamma enhances host resistance against acute disseminated C. albicans infection in mice through activation of polymorphonuclear leukocytes.

MeSH terms

  • Analysis of Variance
  • Animals
  • Blood Cell Count / drug effects
  • CHO Cells
  • Candida albicans / isolation & purification
  • Candidiasis / immunology*
  • Candidiasis / prevention & control
  • Cricetinae
  • Cyclophosphamide / pharmacology
  • Female
  • Hydrocortisone / pharmacology
  • Immunity, Innate
  • Immunosuppression
  • Interferon-gamma / pharmacology*
  • Kidney / microbiology
  • Liver / microbiology
  • Macrophages / immunology
  • Mice
  • Mice, Inbred CBA
  • Neutrophils / immunology
  • Rats
  • Recombinant Proteins
  • Spleen / microbiology

Substances

  • Recombinant Proteins
  • Interferon-gamma
  • Cyclophosphamide
  • Hydrocortisone