Induction of cytotoxic T lymphocytes by recombinant canarypox (ALVAC) and attenuated vaccinia (NYVAC) viruses expressing the HIV-1 envelope glycoprotein

Virology. 1993 Aug;195(2):845-50. doi: 10.1006/viro.1993.1442.


Successful immunization against many viruses, including retroviruses such as HIV-1, is thought to depend upon the roles of both antibody and cytotoxic T-lymphocyte responses. With safety a major concern, we developed two poxvirus recombinants expressing the envelope glycoprotein of HIV-1 IIIB. Canarypox (ALVaC), which is not known to replicate in mammalian cells, and a highly attenuated vaccinia (NYVAC) virus deleted of 18 open reading frames associated with virulence and host range were used as vectors. Upon inoculation into BALB/c mice, both the ALVAC and NYVAC recombinants were capable of inducing antibody responses to HIV gp120 and provoking remarkable levels of primary and memory Thy1.2+, CD4-, CD8+ cytotoxic T-lymphocyte responses to the hypervariable V3 loop of the HIV-1 envelope glycoprotein.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Gene Products, env / genetics
  • Gene Products, env / immunology*
  • Genetic Vectors
  • HIV
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Poxviridae / genetics
  • Poxviridae / immunology*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • T-Lymphocytes, Cytotoxic / immunology*
  • Vaccinia virus / genetics
  • Vaccinia virus / immunology*


  • Gene Products, env
  • Recombinant Proteins