Altered production of PGE2, IL-1 beta and TNF-alpha by peripheral blood monocytes from HIV-positive individuals at early stages of HIV infection

J Acquir Immune Defic Syndr (1988). 1993 Sep;6(9):1017-23.


We analyzed the in vitro synthesis and release of PGE2, IL-1 beta, and TNF-alpha by peripheral blood monocytes from HIV-infected injection drug users at the early clinical stages of HIV infection. We investigated whether there is a concomitant altered production of PGE2 and proinflammatory cytokines by HIV-positive monocytes. We also evaluated T-cell subsets and lymphocyte transformation response to pokeweed mitogen (PWM) in HIV-positive patients and healthy controls. PGE2 and IL-1 beta levels in supernatants from monocyte cultures were determined by radioimmunoassay (RIA), and TNF-alpha by enzyme immunoassay (EIA). Monocytes from asymptomatic HIV-positive individuals produced spontaneous and significantly increased quantities of PGE2, IL-1 beta, and TNF-alpha. Concomitant increased production of PGE2 and IL-1 beta by monocytes from HIV-positive asymptomatic patients was significantly associated with low CD4+ T-cell numbers (< 500 cells/mm3). We also found a strong association between spontaneous and concomitantly increased production of PGE2 and cytokines by monocytes from asymptomatic HIV-positive individuals and a low lymphocyte transformation response to PWM. Further studies are necessary to establish whether this altered production of PGE2 and proinflammatory cytokines by monocytes from HIV-positive individuals might play a role in the mechanisms involved in the progressive impairment of cell-mediated immunity in HIV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Chi-Square Distribution
  • Dinoprostone / biosynthesis*
  • HIV Infections / blood*
  • HIV Infections / etiology
  • HIV Infections / immunology
  • Humans
  • Indomethacin / pharmacology
  • Interleukin-1 / biosynthesis*
  • Lipopolysaccharides / pharmacology
  • Lymphotoxin-alpha / biosynthesis*
  • Monocytes / drug effects
  • Monocytes / immunology
  • Monocytes / metabolism*
  • Substance Abuse, Intravenous / blood
  • Substance Abuse, Intravenous / complications
  • Substance Abuse, Intravenous / immunology


  • Interleukin-1
  • Lipopolysaccharides
  • Lymphotoxin-alpha
  • Dinoprostone
  • Indomethacin