A mitochondrial heat shock protein from Crithidia fasciculata

Mol Biochem Parasitol. 1993 Jun;59(2):191-200. doi: 10.1016/0166-6851(93)90217-l.

Abstract

MCP72 is a mitochondrial hsp70 protein from the trypanosomatid Crithidia fasciculata. An MCP72 cDNA clone was isolated from a C. fasciculata cDNA library by screening with antiserum specific for the homologous protein of Trypanosoma cruzi [9]. The MCP72 cDNA encodes a polypeptide of 663 amino acids which is 84% identical to the Trypanosoma cruzi protein and 56% identical to the Escherichia coli hsp70 protein DnaK. MCP72 is less similar to other hsp70 proteins. Native MCP72 was purified to homogeneity by ATP-agarose affinity chromatography. Comparison of its N-terminal amino acid sequence with that deduced from the cDNA sequence shows that 20 amino acid residues had been cleaved from the N-terminus; this sequence probably represents a mitochondrial import signal which is cleaved during translocation into the mitochondrion. Fluorescence microscopy, using antibodies specific for MCP72, indicates that the protein is concentrated in a region of the mitochondrial matrix which surrounds the kinetoplast.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / biosynthesis
  • Adenosine Triphosphatases / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Crithidia fasciculata / genetics
  • Crithidia fasciculata / metabolism*
  • DNA, Protozoan / genetics
  • DNA, Protozoan / metabolism
  • Gene Library
  • Heat-Shock Proteins / biosynthesis*
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism
  • Mitochondria / metabolism*
  • Molecular Sequence Data
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Trypanosoma cruzi / genetics
  • Trypanosoma cruzi / metabolism

Substances

  • DNA, Protozoan
  • Heat-Shock Proteins
  • Recombinant Proteins
  • Adenosine Triphosphatases

Associated data

  • GENBANK/M95682