Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme

Nucleic Acids Res. 1993 Jul 11;21(14):3281-6. doi: 10.1093/nar/21.14.3281.


The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit from purified holoenzyme, to 6% of total soluble protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Codon
  • DNA Polymerase III / genetics*
  • DNA Polymerase III / metabolism
  • DNA Probes
  • DNA, Bacterial / isolation & purification
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Gene Amplification
  • Genes, Bacterial
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid


  • Codon
  • DNA Probes
  • DNA, Bacterial
  • DNA Polymerase III

Associated data

  • GENBANK/L05381