PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene

Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6859-63. doi: 10.1073/pnas.90.14.6859.

Abstract

cDNAs representing the alpha subunit of polyomavirus enhancer binding protein 2 (PEBP2; also called PEA2) were isolated. The products of the cDNAs are highly homologous to that of Drosophila segmentation gene runt (run) for an N-proximal 128-amino acid region showing 66% identity. The run homology region encompasses the domain capable of binding to a specific nucleotide sequence motif and of dimerizing with the companion beta subunit. The human AML1 gene related to t(8;21) acute myeloid leukemia also had a run homology region. Together with the beta subunit, which increases the affinity of the alpha subunit to DNA without binding to DNA by itself, PEBP2 represents a newly discovered family of transcription factor. The major species of PEBP2 alpha mRNA was expressed in T-cell lines but not in B-cell lines tested. Evidence indicated that PEBP2 functions as a transcriptional activator and is involved in regulation of T-cell-specific gene expression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Core Binding Factor Alpha 2 Subunit
  • Core Binding Factor alpha Subunits
  • DNA-Binding Proteins / genetics*
  • Drosophila / genetics
  • Drosophila Proteins
  • Mice
  • Molecular Sequence Data
  • Multigene Family / genetics*
  • Neoplasm Proteins / genetics*
  • Nuclear Proteins
  • Proto-Oncogene Proteins*
  • RNA, Messenger / genetics
  • Regulatory Sequences, Nucleic Acid
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Transcription Factor AP-2
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*

Substances

  • Core Binding Factor Alpha 2 Subunit
  • Core Binding Factor alpha Subunits
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Neoplasm Proteins
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Runx1 protein, mouse
  • TfAP-2 protein, Drosophila
  • Transcription Factor AP-2
  • Transcription Factors
  • run protein, Drosophila

Associated data

  • GENBANK/D14636
  • GENBANK/D14637