Correlation of antibody titres induced by vaccination with protection in mouse typhoid

Vaccine. 1993;11(7):725-9. doi: 10.1016/0264-410x(93)90256-w.


The ELISA method was used to titrate the humoral immune response in vaccinated mice. When mice were given two doses of a heat-killed salmonella vaccine 6 days apart, there was a steady but low-level increase of antibody synthesis. In contrast, if a booster vaccination was administered 21 days after the primary inoculation, the anamnestic response produced a significantly greater antibody titre, rapidly reaching its peak within 10 days. Such a heightened humoral response induced in the genetically susceptible C57BL/6J mice also coincided with effective protection against an otherwise lethal challenge with Salmonella typhimurium.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood*
  • Bacterial Vaccines / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Immunization Schedule
  • Immunization, Secondary
  • Immunologic Memory
  • Male
  • Mice
  • Mice, Inbred A
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Salmonella Infections, Animal / prevention & control*
  • Salmonella typhimurium / immunology*
  • Vaccination*


  • Antibodies, Bacterial
  • Bacterial Vaccines