Fluid shear stress stimulates platelet-derived growth factor expression in endothelial cells

Am J Physiol. 1993 Jul;265(1 Pt 2):H3-8. doi: 10.1152/ajpheart.1993.265.1.H3.


Fluid flow and the associated shear stress play a critical role in vascular growth and remodeling. Recent data suggest that increased endothelial cell expression of platelet-derived growth factor (PDGF) A- and B-chain by flow may participate in these events. In the present study, we examined the mechanism for flow-induced PDGF expression, focusing on protein kinase C (PKC). Bovine aortic endothelial cells were exposed to flow (shear stress = 30 dyn/cm2) in a parallel-plate flow chamber. Increases in PDGF B-chain, but not PDGF A-chain, were observed within 3 h, maximal within 6 h (13-fold increase), and sustained for 24 h. PKC appeared to be involved because phorbol 12-myristate 13-acetate induced PDGF B-chain mRNA. Activation of PKC alone, however, was insufficient to induce PDGF mRNA because the selective PKC activator, 1-oleoyl-2-acetyl-sn-glycerol, did not induce PDGF expression. A PKC-independent pathway was suggested by the fact that inhibition of PKC (downregulation with phorbol 12,13-dibutyrate or exposure to staurosporine) failed to block PMA or flow-induced PDGF B-chain expression. These results demonstrate flow-induced PDGF B-chain expression in endothelial cells that appears to be mediated, in part, by a PKC-independent pathway.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cells, Cultured
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Endothelium, Vascular / physiology
  • Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
  • Platelet-Derived Growth Factor / chemistry
  • Platelet-Derived Growth Factor / genetics
  • Platelet-Derived Growth Factor / metabolism*
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / physiology
  • Proto-Oncogene Proteins c-fos / genetics
  • RNA, Messenger / metabolism
  • Stress, Mechanical


  • Platelet-Derived Growth Factor
  • Proto-Oncogene Proteins c-fos
  • RNA, Messenger
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Protein Kinase C