Abstract
Invariant chain (Ii) is a nonpolymorphic glycoprotein that associates with major histocompatibility complex class II molecules and has been shown to mediate several functions in class II-restricted antigen presentation. A small proportion of Ii is modified by the addition of chondroitin sulfate (Ii-CS), and this form of Ii is associated with class II on the surface of antigen-presenting cells. In this report we show that expression of Ii-CS dramatically enhanced the ability of class II-positive EL4 transfectants to stimulate class II-dependent allogeneic and mitogenic T cell responses. Antibody blocking studies and the ability of CD44 to bind directly to Ii-CS suggest that Ii-CS can function as an accessory molecule during T cell responses through interactions with CD44.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigen-Presenting Cells / immunology*
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Antigens, Differentiation, B-Lymphocyte*
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Biological Transport
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Cells, Cultured
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Chondroitin Sulfates / immunology*
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Glycoproteins / immunology
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Glycoproteins / metabolism
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Histocompatibility Antigens Class II / immunology*
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Histocompatibility Antigens Class II / metabolism
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Lymph Nodes / immunology
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Lymphocyte Activation
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Mesentery / immunology
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Mice
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Mice, Inbred C57BL
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Protein Folding
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Receptors, Lymphocyte Homing / immunology*
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Receptors, Lymphocyte Homing / metabolism
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T-Lymphocytes / immunology*
Substances
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Antigens, Differentiation, B-Lymphocyte
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Glycoproteins
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Histocompatibility Antigens Class II
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Receptors, Lymphocyte Homing
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invariant chain
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Chondroitin Sulfates