Abstract
Fission yeast cut5 mutants cause cytokinesis in the absence of normal nuclear division. We show here that cut5+ is required for both the onset of S phase and the restraint of M phase before the completion of S phase. The primary defects in cut5 mutants occur prior to S phase, but cells suffer lethal damage during M phase. Mitosis and cytokinesis occur in the presence of hydroxyurea or in the double mutant cdc10-cut5 (the cdc10 mutation alone blocks progression from G1 to S). Gene cloning shows that cut5+ is identical to the fission yeast rad4+ gene, which is similar to human XRCC1. The rad4+/cut5+ gene is unique in its positive role for replication/repair and in its negative role for mitosis/cytokinesis. We propose a single/twin chromatid marking model for rad4+/cut5+ function in cell cycle control.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Cycle / genetics*
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Cell Cycle Proteins*
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Cell Division / genetics
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Cell Nucleus
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Cloning, Molecular
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DNA, Fungal / biosynthesis
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DNA-Binding Proteins*
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Fungal Proteins / genetics*
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Genes, Fungal / genetics*
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Genes, Fungal / radiation effects
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Mitosis / genetics
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Models, Genetic
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Molecular Sequence Data
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Nitrogen / deficiency
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Phenotype
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S Phase / genetics
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Schizosaccharomyces / genetics*
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Schizosaccharomyces / radiation effects
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Schizosaccharomyces pombe Proteins*
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Transcription Factors
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Transformation, Genetic
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Transglutaminases*
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Ultraviolet Rays / adverse effects
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X-Rays / adverse effects
Substances
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Cell Cycle Proteins
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DNA, Fungal
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DNA-Binding Proteins
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Fungal Proteins
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RAD4 protein, S pombe
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Schizosaccharomyces pombe Proteins
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Transcription Factors
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cdc10 protein, S pombe
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Transglutaminases
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Nitrogen
Associated data
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GENBANK/D16627
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GENBANK/L14569
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GENBANK/L19248
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GENBANK/L19249
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GENBANK/L33709
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GENBANK/S60902
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GENBANK/S60903
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GENBANK/S60904
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GENBANK/S60905
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GENBANK/S60924