Fission yeast cut5+, required for S phase onset and M phase restraint, is identical to the radiation-damage repair gene rad4+

Cell. 1993 Jul 30;74(2):383-93. doi: 10.1016/0092-8674(93)90428-s.


Fission yeast cut5 mutants cause cytokinesis in the absence of normal nuclear division. We show here that cut5+ is required for both the onset of S phase and the restraint of M phase before the completion of S phase. The primary defects in cut5 mutants occur prior to S phase, but cells suffer lethal damage during M phase. Mitosis and cytokinesis occur in the presence of hydroxyurea or in the double mutant cdc10-cut5 (the cdc10 mutation alone blocks progression from G1 to S). Gene cloning shows that cut5+ is identical to the fission yeast rad4+ gene, which is similar to human XRCC1. The rad4+/cut5+ gene is unique in its positive role for replication/repair and in its negative role for mitosis/cytokinesis. We propose a single/twin chromatid marking model for rad4+/cut5+ function in cell cycle control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle / genetics*
  • Cell Cycle Proteins*
  • Cell Division / genetics
  • Cell Nucleus
  • Cloning, Molecular
  • DNA, Fungal / biosynthesis
  • DNA-Binding Proteins*
  • Fungal Proteins / genetics*
  • Genes, Fungal / genetics*
  • Genes, Fungal / radiation effects
  • Mitosis / genetics
  • Models, Genetic
  • Molecular Sequence Data
  • Nitrogen / deficiency
  • Phenotype
  • S Phase / genetics
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / radiation effects
  • Schizosaccharomyces pombe Proteins*
  • Transcription Factors
  • Transformation, Genetic
  • Transglutaminases*
  • Ultraviolet Rays / adverse effects
  • X-Rays / adverse effects


  • Cell Cycle Proteins
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • RAD4 protein, S pombe
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • cdc10 protein, S pombe
  • Transglutaminases
  • Nitrogen

Associated data

  • GENBANK/D16627
  • GENBANK/L14569
  • GENBANK/L19248
  • GENBANK/L19249
  • GENBANK/L33709
  • GENBANK/S60902
  • GENBANK/S60903
  • GENBANK/S60904
  • GENBANK/S60905
  • GENBANK/S60924